After MiSeq launched, Illumina made significant advancements in sequencing chemistry and wanted to bring the power to sequence a whole human genome to the bench top as well. Along with this new instrument, I wanted to improve upon some of the workflow issues I had seen after MiSeq launched:
As with the MiSeq project, I did extensive ethnographic research at the beginning of the project. From this and my experience with MiSeq, I realized that the focus of my work should not be on the sequencing software (which was already quite good from MiSeq) but on the non-instrument parts of the workflow. By presenting my findings to stakeholders, I was able to strategically pull groups together from different departments and secure funding for the development of a program that wasn't even on the roadmap.
This new program was arranged to support both the development of an on-site analysis server running the BaseSpace software and to add in functionality to set up runs directly in BaseSpace and monitor them remotely. Once this was built, the workflow would be:
Unfortunately, I was not able to see this project through to completion since I left the company for a new opportunity. However, the products I laid the groundwork for were all developed and integrated into the sequencing workflow which launched over a year after I left.Watch a video about the NextSeq 500